Thesis Seminare

Hello!
My recent Thesis Seminare!
Calcium Mediated Signaling in Diastolic Heart Failure

Study of growth property of complement C2 produced by THP-1 cells using the chemical agents –Histamine, Imidazole and β- Glycyrrhetinic acid

ABSTRACT:

Despite significant research on the role of inflammation and immunosurveillance in the immunologic microenvironment of cancer, little attention has been given to the oncogenic capabilities of the complement cascade. The complement system is the major branch of the humoral immune system.  Complement components C2 and factor B are essential for the classical and alternative pathways of complement activation, respectively, because they provide catalytic subunits for the C3 and C5 convertases, both of which are key enzymes for the complement system. Monocytes and macrophages synthesize complement proteins, thus providing an essential local source of these proteins in vivo which serve as a first-line host defense mechanism. The recent findings have shown that complement factor especially Factor B and C2 facilitates cellular proliferation and regeneration. We address this hypothesis using chemical agents Histamine, Imidazole and β- Glycyrrhetinic acid who individually influence the factor C2 production in THP-1 cells (human monocytic leukemia cell line). We investigated the growth property of complement C2 by studying the effect of these three chemical agents on the growth of THP-1 cells in time and concentration dependent manner. In this study, to our knowledge we have shown that the Complement C2 has a proliferating effect on monocytic leukemia cells. Additionally, the results show that these three chemical agents do have an influence on each other’s growth properties. Given that the traditionally held functions for the complement system include innate immunity and cancer defense, our study suggests a new way of thinking about the role of complement proteins in monocytes especially human monocytic leukemia. These results may give us leads for the treatment of monocytic leukaemias like AML-M5 which are difficult to treat.

Key words: Cancer, Complement C2, Factor B, Histamine, Imidazole and β- Glycyrrhetinic acid, THP-1, AML-M5.

For the whole article click on the link below:

https://docs.google.com/open?id=0BzdHWb7McaBDRG0tNmNFTGg2Tzg 

Diuretics- Treatment of Congestive Heart Failure

In Cardiovascular disease, the heart of the patient cannot pump enough oxygen and nutrients to meet the body's needs. The chambers of the heart respond by stretching to hold more blood to pump through the body or by becoming stiff and thickened. This helps to keep the blood moving for a short while but, in time, the heart muscle walls weaken and are unable to pump as strongly (2). As a result, the kidneys often respond by causing the body to retain fluid and sodium. If fluid builds up in the organs, the body becomes congested, and congestive heart failure (CHF) is the term used to describe the condition (1). CHF is the cause of significant morbimortality all over the world and its incidence and prevalence are rising. There are a variety of therapy options for CHF, but diuretics play an important role (Acuña C. et. al., 2010).

For futher information click on the link below:

Diuretics: Treatment of Congestive Heart Failure